Biocide100 Efficacy Data

DETERGENT DISINFECTANT and PUMP SPRAY (DDPS)

(EPA Reg. No. 86117)

EFFICACY DATA for: BioCide 100

VIRUCIDAL DATA:

Test Methods:

U.S. E.P.A. Pesticide Assessment Guidelines. Subdivision G: Product Performance, Section 91-2(f)~and Section 91-30 (d),
(e), November, 1982.

Protocols for Testing the Efficacy of Disinfectants against Hepatitis B Virus (HBV) (EPA. Federal Register, Vol. 65, No.
166, 812512000,p. 51828).

Protocol for Testing Disinfectants against Hepatitis C Virus using Bovine Viral Diarrhea Virus as approved by the U.S.
EPA on August 15,2002.

Modified U.S. E.P.A. Pesticide Assessment Guidelines, Subdivision G: Product Performance, Section 91-2(f), and Section
91-30 (d), (e), November, 1982.

Test Conditions: ready-to-use (RTU), organic soil load. room temperature, glass petri dish substrates

Results:

Avian Inf1uenzaA Virus (H3N2) (Avian Reassortant) (ATCC VR-2072)
A&B
>3.0 log10
2 minutes
Avian Influenza Virus, Type A (Turkey/W1S/66) (H9N2)
A&B
>4.83 log10
2 minutes
Bovine Viral Diarrhea Virus (BVDV)
A&B
>3.0 log10
5 minutes
Canine Parvovirus (ATCC VR-20l7)
A&B
>3.0 log10
10 minutes
Feline Calicivirus (FCV)
A&B
>6.48 log10
30 seconds
Hepatitis A Virus (HAV)
A&B
>3.0 log10
10 minutes
Hepatitis B Virus (HBV) (Duck.Hepatitis B Virus-DHBV)
A&B
>33 log10
5 minutes
Hepatitis C Virus (HCV) (Bovine Viral Diarrhea Virus-BVDV)
A&B
>3.0 log10
5 minutes
Human immunodeficiency Virus, HTLV-IIIRF, strain of HIV-1 (associated with AIDS)
A&B
>3.5 log10
1 minute
Human Coronavirus (ATCC VR-740. strain 229E)
A&B
>3.0 log10
2 minutes
Nuruvirus (Nurwalk Virus)
A&B
>6.48 log10
30 seconds
Paramyxovirus (Mumps) (ATCC VR-1438)
A&B
>3.0 log10
3 minutes
Poliovirus Type I, strain Brunhilde (ATCC VR-1000)
A&B
>3.25 log10
10 minutes
Rabies Virus (attenuated ERA strain, CDC)
A&B
>3.0 log10
30 seconds
Rhinovirus Type 39 (ATCC VR-340)
A&B
>3.0 log10
3 minutes
Rotovirus
A&B
>3.0 log10
3 minutes
SARS Associated Coronavirus (ZeptoMetrix)
A&B
>4.03 log10
2 minutes

Conclusion: Under the conditions of this investigation. BioCide 100 was virucidal for Avian Influenza A Virus (H3N2). Avian Influenza Virus Type A (H9N2), Bovine Viral Diarrhea Virus (BVDV), Canine Parvovirus, Feline Calicivirus (FCV), Hepatitis A Virus (HAV), Hepatitis B Virus (HBV), Hepatitis C Virus (HCV), Human Immunodeficiency Virus (HIV-1), Human Coronavirus, Norovirus (Norwallc Virus), Paramyxovirus (Mumps), Poliovirus Type 1, Rabies, Rhinovirus Type 39, Rotovirus, and SARS Associated Coronavirus according to criteria established by the U_S. Environmental Protection Agency for registration and labeling of a disinfectant product as a virucide.
EFF-083-08

BioCide Labs

BioCide 100

DETERGENT DISINFECTANT and PUMP SPRAY
EPA No. 86117

TUBERCULOCIDAL DATA:

Test Method:
AOAC Confinnative In Vitro Test for Determining Tuberculocidal Activity
Test Organism:
Mycobacterium bovis BCG
Test Conditions:
ready-to-use (RTU), organic soil load, 5 minute contact time, glass slide carrier substrates

Results:

Subculture Media
Sample
No. of Exposed
No. of Carriers
Carriers Showing Growth
modified Proskauer-Beek Medium
A
10
0
 
B
10
0
Middlebrook 7H9 Broth
A
10
0
 
B
10
0
Kirchners Medium
A
10
0
 
B
10
0
Conclusion: Under the conditions of this investigation. BioCide 100 was tuberculocidal for Mycobacterium bovis (BCG) according to criteria established by the U.S. Environmental Protection Agency for registration and labeling of a disinfectant product as a tuberculocide.
MILDEW FUNGISTATIC DATA:
Test Method:
EPA Hard Surface Mildew Fungistatic Test
Test Organism:
Aspergillus niger (ATCC 6275)
Test Conditions:
glazed ceramic tile substrates
Results:
 
Sample
No. of Exposed Tiles
No. of Tiles Showing Growth
DDPS
10
0
Control
10
10
Conclusion: Under the conditions of this investigation. BioCide 100 was fungistatic for Aspergillus niger according to criteria established by the U. S. Environmental Protection Agency for registration and labeling of a disinfectant product as a fungistat.
FUNGICIDAL DATA:
Test Method:
AOAC Germicidal Spray Products as Disinfectants
Test Conditions:
ready-to-use (RTU), organic soil load, room temperature, glass slide carrier substrates
Results:
 
Organism
Sample
Exposed
Positive
Contact Time
Trichophyton mentagrophytes (ATCC 9533)
A
60
0
10 minutes
 
B
60
0
 
 
C
60
0
 
Conclusion: Under the conditions of this investigation, BioCide 100 was fungicidal for Trichophyton menfagrophytes according to criteria established by the U. S. Environmental Protection Agency for registration and labeling of a disinfectant product as a fungicide.

BioCide Labs

BioCide 100

DETERGENT DISINFECTANT and PUMP SPRAY
EPA No. 86117
BACTERICIDAL DATA:
Test Method:
AOAC Germicidal Spray Products as Disinfectants
Test Conditions:
ready-to-use (RTU). organic soil load. room temperature, glass slide carrier substrates
Results:
 
Organism
Sample
Exposed
Positive
Contact Time
Stphylococcus aureus (ATCC 6538)
A
60
0
3 minutes
 
B
60
1
 
Salmonella (cboleraesuis) enterica (ATCC 10708)
A
60
0
3 minutes
 
B
60
0
 
Pseudomonas aeruginosa (ATCC 15442)
A
60
0
3 minutes
 
B
60
0
 
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