BioCide 100 Efficacy Data
DETERGENT DISINFECTANT and PUMP SPRAY (DDPS)
VIRUCIDAL DATA:
Test Methods:
U.S. E.P.A. Pesticide Assessment Guidelines. Subdivision G: Product Performance, Section 91-2(f)~and Section 91-30 (d), (e), November, 1982.
Protocols for Testing the Efficacy of Disinfectants against Hepatitis B Virus (HBV) (EPA. Federal Register, Vol. 65, No. 166, 812512000,p. 51828).
Protocol for Testing Disinfectants against Hepatitis C Virus using Bovine Viral Diarrhea Virus as approved by the U.S. EPA on August 15,2002.
Modified U.S. E.P.A. Pesticide Assessment Guidelines, Subdivision G: Product Performance, Section 91-2(f), and Section 91-30 (d), (e), November, 1982.
Test Conditions:
ready-to-use (RTU), organic soil load. room temperature, glass petri dish substrates
Results:
Avian Inf1uenzaA Virus (H3N2) (Avian Reassortant) (ATCC VR-2072)A&B>3.0 log102 minutes
Avian Influenza Virus, Type A (Turkey/W1S/66) (H9N2)A&B>4.83 log102 minutes
Bovine Viral Diarrhea Virus (BVDV)A&B>3.0 log105 minutes
Canine Parvovirus (ATCC VR-20l7)A&B>3.0 log1010 minutes
Feline Calicivirus (FCV)A&B>6.48 log1030 seconds
Hepatitis A Virus (HAV)A&B>3.0 log1010 minutes
Hepatitis B Virus (HBV) (Duck.Hepatitis B Virus-DHBV)A&B>33 log105 minutes
Hepatitis C Virus (HCV) (Bovine Viral Diarrhea Virus-BVDV)A&B>3.0 log105 minutes
Human immunodeficiency Virus, HTLV-IIIRF, strain of HIV-1 (associated with AIDS)A&B>3.5 log101 minute
Human Coronavirus (ATCC VR-740. strain 229E)A&B>3.0 log102 minutes
Nuruvirus (Nurwalk Virus)A&B>6.48 log1030 seconds
Paramyxovirus (Mumps) (ATCC VR-1438)A&B>3.0 log103 minutes
Poliovirus Type I, strain Brunhilde (ATCC VR-1000)A&B>3.25 log1010 minutes
Rabies Virus (attenuated ERA strain, CDC)A&B>3.0 log1030 seconds
Rhinovirus Type 39 (ATCC VR-340)A&B>3.0 log103 minutes
RotovirusA&B>3.0 log103 minutes
SARS Associated Coronavirus (ZeptoMetrix)A&B>4.03 log102 minutes
Conclusion:
Under the conditions of this investigation. BioCide 100 was virucidal for Avian Influenza A Virus (H3N2). Avian Influenza Virus Type A (H9N2), Bovine Viral Diarrhea Virus (BVDV), Canine Parvovirus, Feline Calicivirus (FCV), Hepatitis A Virus (HAV), Hepatitis B Virus (HBV), Hepatitis C Virus (HCV), Human Immunodeficiency Virus (HIV-1), Human Coronavirus, Norovirus (Norwallc Virus), Paramyxovirus (Mumps), Poliovirus Type 1, Rabies, Rhinovirus Type 39, Rotovirus, and SARS Associated Coronavirus according to criteria established by the U_S. Environmental Protection Agency for registration and labeling of a disinfectant product as a virucide.
EFF-083-08
Biocide Labs – BioCide 100
DETERGENT DISINFECTANT and PUMP SPRAY
EPA No. 86117
TUBERCULOCIDAL DATA:
Test Method:
AOAC Confinnative In Vitro Test for Determining Tuberculocidal Activity
Test Organism:
Mycobacterium bovis BCG
Test Conditions:
ready-to-use (RTU), organic soil load, 5 minute contact time, glass slide carrier substrates
| Results: | |||
| Subculture Media | Sample | No. of Exposed | No. of Carriers |
| Carriers Showing Growth | |||
| modified Psoskauer-Beek Medium | A | 10 | 0 |
| B | 10 | 0 | |
| Middlebrook 7H9 Broth | A | 10 | 0 |
| B | 10 | 0 | |
| Kirchners Medium | A | 10 | 0 |
| B | 10 | 0 |
MILDEW FUNGISTATIC DATA:
Test Method:
EPA Hard Surface Mildew Fungistatic Test
Test Organism:
Aspergillus niger (ATCC 6275)
Test Conditions:
glazed ceramic tile substrates
| Results: | ||
| Sample | No. of Exposed | No. of Tiles Showing Growth |
| DDPS | 10 | 0 |
| Control | 10 | 10 |
FUNGICIDAL DATA:
Test Method:
AOAC Germicidal Spray Products as Disinfectants
Test Conditions:
ready-to-use (RTU), organic soil load, room temperature, glass slide carrier substrates
| Results: | ||||
| Organism | Sample | Exposed | Positive | Contact Time |
| Trichophyton mentagrophytes | A | 60 | 0 | 10 Minutes |
| B | 60 | 0 | ||
| Middlebrook 7H9 Broth | C | 60 | 0 |
Biocide Labs – BioCide 100
DETERGENT DISINFECTANT and PUMP SPRAY
EPA No. 86117
BACTERICIDAL DATA:
Test Method:
AOAC Germicidal Spray Products as Disinfectants
Test Conditions:
ready-to-use (RTU), organic soil load, room temperature, glass slide carrier substrates
| Results: | ||||
| Organism | Sample | Exposed | Positive | Contact Time |
| Stphylococcus aureus (ATCC 6538) | A | 60 | 0 | 3 Minutes |
| B | 60 | 1 | ||
| Salmonella (cboleraesuis) enterica (ATCC 10708) | A | 60 | 0 | 3 Minutes |
| B | 60 | 0 | ||
| Pseudomonas aeruginosa (ATCC 15442) | A | 60 | 0 | 3 Minutes |
| B | 60 | 0 |
