Biocide100 Efficacy Data

                                           Biocide 100 Efficacy  Data

DETERGENT DISINFECTANT and PUMP SPRAY (DDPS)

(EPA Reg. No. 86117)

EFFICACY DATA for: BioCide 100


VIRUCIDAL DATA:

Test Methods:

U.S. E.P.A. Pesticide Assessment Guidelines. Subdivision G: Product Performance, Section 91-2(f)~and Section 91-30 (d),

(e), November, 1982.

Protocols for Testing the Efficacy of Disinfectants against Hepatitis B Virus (HBV) (EPA. Federal Register, Vol. 65, No.

166, 812512000,p. 51828).

Protocol for Testing Disinfectants against Hepatitis C Virus using Bovine Viral Diarrhea Virus as approved by the U.S.

EPA on August 15,2002.

Modified U.S. E.P.A. Pesticide Assessment Guidelines, Subdivision G: Product Performance, Section 91-2(f), and Section

91-30 (d), (e), November, 1982.

Test Conditions: ready-to-use (RTU), organic soil load. room temperature, glass petri dish substrates


Results:


 Avian Inf1uenzaA Virus (H3N2) (Avian Reassortant) (ATCC VR-2072)  A&B >3.0 log10 2 minutes
 Avian Influenza Virus, Type A (Turkey/W1S/66) (H9N2)
 A&B >4.83 log10
2 minutes
 Bovine Viral Diarrhea Virus (BVDV)  A&B >3.0 log10 5 minutes 
 Canine Parvovirus (ATCC VR-20l7)  A&B >3.0 log10 10 minutes
 Feline Calicivirus (FCV)  A&B >6.48 log10 30 seconds
 Hepatitis A Virus (HAV)  A&B >3.0 log10 10 minutes
 Hepatitis B Virus (HBV) (Duck.Hepatitis B Virus-DHBV)  A&B >33 log10 5 minutes
 Hepatitis C Virus (HCV) (Bovine Viral Diarrhea Virus-BVDV)   A&B >3.0 log10 5 minutes
 Human immunodeficiency Virus, HTLV-IIIRF, strain of HIV-1  (associated with AIDS  A&B >3.5 log10 1 minute
 Human Coronavirus (ATCC VR-740. strain 229E)   A&B >3.0 log10 2 minutes
 Nuruvirus (Nurwalk Virus)  A&B >6.48 log10 30 seconds
 Paramyxovirus (Mumps) (ATCC VR-1438)  A&B >3.0 log10 3 minutes
 Poliovirus Type I, strain Brunhilde (ATCC VR-1000)  A&B >3.25 log10 10 minutes
 Rabies Virus (attenuated ERA strain, CDC)  A&B >3.0 log10 30 seconds
 Rhinovirus Type 39 (ATCC VR-340)  A&B >3.0 log10 3 minutes
 Rotovirus  A&B >3.0 log10 3 minutes
 SARS Associated Coronavirus (ZeptoMetrix)  A&B >4.03 log10 2 minutes

Conclusion: Under the conditions of this investigation. BioCide 100 was virucidal for Avian Influenza A Virus (H3N2). Avian Influenza Virus Type A (H9N2), Bovine Viral Diarrhea Virus (BVDV), Canine Parvovirus, Feline Calicivirus (FCV), Hepatitis A Virus (HAV), Hepatitis B Virus (HBV), Hepatitis C Virus (HCV), Human Immunodeficiency Virus (HIV-1), Human Coronavirus, Norovirus (Norwallc Virus), Paramyxovirus (Mumps), Poliovirus Type 1, Rabies, Rhinovirus Type 39, Rotovirus, and SARS Associated Coronavirus according to criteria established by the U_S. Environmental Protection Agency for registration and labeling of a disinfectant product as a virucide.

EFF-083-08


 

 

BioCide Labs

BioCide 100

DETERGENT DISINFECTANT and PUMP SPRAY

EPA No. 86117

 

TUBERCULOCIDAL DATA:

 

Test Method:                     AOAC Confinnative In Vitro Test for Determining Tuberculocidal Activity

Test Organism:                 Mycobacterium bovis BCG

Test Conditions:               ready-to-use (RTU), organic soil load, 5 minute contact time, glass slide carrier substrates

Results:

 Subculture Media  Sample   No. of Exposed          No. of Carriers
Carriers Showing Growth
 modified Proskauer-Beek Medium     A       10
           0

      B
      10
           0
 Middlebrook 7H9 Broth     A       10            0

    B       10            0
 Kirchners Medium     A       10            0

    B       10            0
           

Conclusion: Under the conditions of this investigation. BioCide 100 was tuberculocidal for Mycobacterium bovis (BCG) according to criteria established by the U.S. Environmental Protection Agency for registration and labeling of a disinfectant product as a tuberculocide.

 

MILDEW FUNGISTATIC DATA:

Test Method:                       EPA Hard Surface Mildew Fungistatic Test

Test Organism:                    Aspergillus niger (ATCC 6275)

Test Conditions:                   glazed ceramic tile substrates

Results:

 Sample
 No. of Exposed Tiles  No. of Tiles Showing Growth
 DDPS            10               0
 Control            10              10
 

Conclusion: Under the conditions of this investigation. BioCide 100 was fungistatic for Aspergillus niger according to criteria established by the U. S. Environmental Protection Agency for registration and labeling of a disinfectant product as a fungistat.

FUNGICIDAL DATA:

Test Method:      AOAC Germicidal Spray Products as Disinfectants

Test Conditions:     ready-to-use (RTU), organic soil load, room temperature, glass slide carrier substrates

Results:

 Organism  Sample   Exposed
  Positive  Contact Time
 Trichophyton mentagrophytes (ATCC 9533)
 A      60       0  10 minutes

 B      60       0

 C
     60       0

 

 

Conclusion: Under the conditions of this investigation, BioCide 100 was fungicidal for Trichophyton menfagrophytes according to criteria established by the U. S. Environmental Protection Agency for registration and labeling of a disinfectant product as a fungicide.

                                              

BioCide Labs

BioCide 100

DETERGENT DISINFECTANT and PUMP SPRAY

EPA No. 86117

BACTERICIDAL DATA:

Test Method:             AOAC Germicidal Spray Products as Disinfectants

Test Conditions:        ready-to-use (RTU). organic soil load. room temperature, glass slide carrier substrates


Results:


 Organism  Sample  Exposed  Positive  Contact Time
 Stphylococcus aureus (ATCC 6538)  A  60  0  3 minutes
   B  60  1  
 Salmonella (cboleraesuis) enterica (ATCC 10708)  A  60  0  3 minutes
   B  60  0  
 Pseudomonas aeruginosa (ATCC 15442)  A  60  0  3 minutes
   B  60  0  

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